misprime

verb

misprime

verb

Etymology From mis- + prime.

1

To prime incorrectly.

It is also useful when using degenerate primers which may misprime and cause nonspecific products.2007, Ralph Rapley, John M. Walker, Molecular Biomethods Handbook, page 310:
As described previously, primers are apt to dimerize or misprime, particularly in the absence of specific target.2010, Stephen A. Bustin, The PCR Revolution: Basic Technologies and Applications, page 139:

1 more example

Aberrant annealing has also been observed — either due to the use of the incorrect tRNA to misprime reverse transcription (Colicelli and Goff 1986a, b; Wakefield et al. 1995) or due to the priming of cellular mRNAs with short “tRNA-like” primers (Taylor and Cywinski 1984).2013, Stuart LeGrice, Matthias Gotte, Human Immunodeficiency Virus Reverse Transcriptase, page 208:

Entry derived from the Wiktionary, under licence CC BY-SA 4.0 — list of authors.