biotinylate
verbEtymology From biotinyl + -ate.
1
To attach a biotin residue to a biological macromolecule in order to label it
- After 30 min, the reaction was supplemented to a final volume of 25 μL with 5 units of Klenow fragment (3′→5′ exo-) DNA polymerase (NEB, USA) and 5 μM of biotin-14-dUTP, dATP, dGTP and dCTP and incubated at 37 °C for another 30 min to fill in the overhangs and biotinylate the fragmented DNA.2015 July 11, Eugene J. H. Wee, Thu Ha Ngo, Matt Trau, “Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs”, in Clinical Epigenetics, volume 7, →DOI:
- Derived terms
- autobiotinylate
- Related terms
- biotinylation